We devised a DNA nanothermometry strategy involving three strands of DNA: an anchor strand that binds to the surface of our nanoparticle (blue), a blocking strand that hybridizes to the anchor strand (orange), and an imaging strand that is complementary to the end of the blocking strand and also carries a fluorescent molecule.  By monitoring individual fluorescent events associated with binding of the imaging strand, we create temperature-dependent binding curves.  We also use super-resolution imaging to reconstruct the images of the underlying nanoparticles.